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# pH Buffer Solution Mixing Tutorial

pH Meters require calibration with Buffer Solutions, follow this tutorial to mix the required solutions.

<figure><img src="https://files.gitbook.com/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F6uJvpXC43onNIIwhMlWo%2Fuploads%2FBfxMH8n51jiISWczn8o3%2F20240729_132754.jpg?alt=media&#x26;token=32177461-fdd2-4150-b2d1-b02fd53b9fed" alt=""><figcaption></figcaption></figure>

Prepare the following items:

* 250mL Bottle \*3
* Buffer Solution Powder(pH 4.00, pH 6.86, pH 9.18 each)
* Buffer Solution Labels

pH 6.86 Buffer Solution is shown as an example here.

### Mixing the Solution

Clean the plastic bottle with water and wipe dry completely.

Pour the powder into the bottle.

<figure><img src="https://files.gitbook.com/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F6uJvpXC43onNIIwhMlWo%2Fuploads%2FZ06l3qQlFizj8ZMjSJM6%2F20240729_132950.jpg?alt=media&#x26;token=642a2dba-44d1-4bac-ae99-f4dab31f059f" alt=""><figcaption></figcaption></figure>

Add 250mL of distilled water into the bottle, also wash the packet with a little bit of distilled water to dissolve all the remaining powder and then pour it into the bottle.

<figure><img src="https://files.gitbook.com/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F6uJvpXC43onNIIwhMlWo%2Fuploads%2FfrSYW1IVs1X1uX9ZrCb3%2F20240729_133213.jpg?alt=media&#x26;token=2c21e45d-90df-4b47-a993-adeffc04e005" alt=""><figcaption></figcaption></figure>

Close the bottle cap tightly and shake thoroughly, make sure all powder is dissolved. Put on a label to identify.

<figure><img src="https://files.gitbook.com/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F6uJvpXC43onNIIwhMlWo%2Fuploads%2F6NHfwipJm17PCd6oFPp4%2F20240729_133259.jpg?alt=media&#x26;token=89b5f71e-4177-4ccf-866b-a2378da794e8" alt=""><figcaption></figcaption></figure>

### Usage Guide for Buffer Solution

* In order to maintain the quality of the solution, avoid leaving the cap open and expose the solution in the air for long periods of time.
* It is recommended to simply pour out some solution and discard after using, rather than use the entire bottle for calibration.

### Safety Precautions

Although the powder in the packets are not toxic and harmful to humans, it is still recommended to handle with care.

* Avoid ingesting: The powders are non-toxic, but ingestion may lead to diarrhea.
* Avoid touching: The powders are non-toxic, but touching may lead to skin irritation.
